Wright State University View Institution's Website 10 articles published in JoVE Bioengineering Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy Chien Poon1, Ben Rinehart1, Jun Li1,2, Ulas Sunar1 1Department of Biomedical, Industrial and Human Factors Engineering, Wright State University, 2South China Academy of Advanced Optoelectronics, South China Normal University This protocol demonstrates how to measure resting state functional connectivity in the human prefrontal cortex using a custom-made diffuse correlation spectroscopy instrument. The report also discuss practical aspects of the experiment as well as detailed steps for analyzing the data. Biology Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry Victoria Wong1, Prakash Arumugam1, Lucile E. Wrenshall1,2 1Department of Neuroscience, Cell Biology, and Physiology, Wright State University, 2Department of Surgery, Boonshoft School of Medicine, Wright State University Proliferation is a critical part of cellular function, and a common readout used to assess potential toxicity of new drugs. Measuring proliferation is, therefore, a frequently used assay in cell biology. Here we present a simple, versatile method of measuring proliferation that can be used in adherent and non-adherent cells. Genetics Mating-based Overexpression Library Screening in Yeast Elliott Hayden1, Shuzhen Chen1, Abagail Chumley1, Quan Zhong1, Shulin Ju1 1Department of Biological Sciences, Wright State University This article presents a mating-based method to facilitate overexpression screening in budding yeast using an arrayed plasmid library. Neuroscience Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions Steven R. A. Burke1, Eric J. Reed1, Shannon H. Romer1, Andrew A. Voss1 1Department of Biological Sciences, Wright State University The protocol described in this paper uses the mouse levator auris longus (LAL) muscle to record spontaneous and nerve-evoked postsynaptic potentials (current-clamp) and currents (voltage-clamp) at the neuromuscular junction. Use of this technique can provide key insights into mechanisms of synaptic transmission under normal and disease conditions. Neuroscience A Protocol for the Administration of Real-Time fMRI Neurofeedback Training Matthew S. Sherwood1,2, Emily E. Diller2, Elizabeth Ey3, Subhashini Ganapathy2,4, Jeremy T. Nelson5, Jason G. Parker1,6 1Office of the Vice President for Research and Graduate Studies, Wright State University, 2Department of Biomedical, Industrial and Human Factors Engineering, Wright State University, 3 The ability to induce and/or control neural plasticity may be critical in future treatments for neurologic disorders and the recovery from brain injury. In this paper, we present a protocol on the use of neurofeedback training with functional magnetic resonance imaging to modulate human brain function. Medicine Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease Victoria N. Jensen*1, Shannon H. Romer*2, Sarah M. Turner3, Steven A. Crone3 1Neuroscience Graduate Program, University of Cincinnati, 2Department of Biological Sciences, Wright State University, 3 This paper introduces a method for repeated measurements of ventilation and respiratory muscle activity in a freely behaving amyotrophic lateral sclerosis (ALS) mouse model throughout disease progression with whole-body plethysmography and electromyography via an implanted telemetry device. Immunology and Infection Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs Jennifer N. Gibson1, Pavani Beesetty1, Courtney Sulentic2, J. Ashot Kozak1 1Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine, Wright State University, 2Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters. Neuroscience Olfactory Assays for Mouse Models of Neurodegenerative Disease Andrew M. Lehmkuhl1, Emily R. Dirr2,3, Sheila M. Fleming1,2 1Department of Psychology, University of Cincinnati, 2Department of Neurology, University of Cincinnati, 3Department of Neuroscience, Cell Biology, and Physiology, Wright State University Impairment in olfactory function is a common feature in many neurodegenerative disorders including Parkinson, Alzheimer, and Huntington diseases. In the present article, we describe a set of tests for assessing olfaction discrimination and detection in mice that can be used to measure olfactory abilities in mouse models of neurodegenerative diseases. Biology Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles Catherine B. Anders1, Joshua D. Baker1, Adam C. Stahler1, Austin J. Williams1, Jackie N. Sisco2, John C. Trefry2, Dawn P. Wooley2, Ioana E. Pavel Sizemore1 1Department of Chemistry, Wright State University, 2Department of Neuroscience, Cell Biology, and Physiology, Wright State University Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation. Neuroscience Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats Saeed Alshahrani1, Filipe Fernandez-Conti2, Amanda Araujo2, Mauricio DiFulvio1 1Department of Pharmacology and Toxicology, Wright State University, 2Human Movement Laboratory, Universidade São Judas Tadeu Here, we describe a rapid reliable and simple procedure to determine the lowest temperature at which rats or mice show nocifensive behavior, i.e. the thermal nociceptive threshold (TNT). This method applies a slowly increasing thermal stimulus allowing precise and reproducible estimation of TNTs with minimum, if any, stress to the animals.