Universidade Federal de Santa Catarina 1 article published in JoVE Biology Bone Conditioned Medium: Preparation and Bioassay Jordi Caballé-Serrano1,2,3, Kosaku Sawada2,5, Guenther Schuldt Filho1,2,6, Dieter D. Bosshardt1,4, Daniel Buser1, Reinhard Gruber1,2 1Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, 2Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, 3Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Universitat Internacional de Catalunya, 4Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern, 5Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, 6Department of Implant Dentistry, School of Dentistry, Universidade Federal de Santa Catarina We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.