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Biologia
Propagation of Human Embryonic Stem (ES) Cells
Published: November 30, 2006
doi:
10.3791/119
Laurence Daheron
1
Center for Regenerative Medicine
,
MGH – Massachusetts General Hospital
Riferimenti
Kawasaki, H.
Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.
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, 31-40 (2000).
Perrier, A. L.
Derivation of midbrain dopamine neurons from human embryonic stem cells.
Proc Natl Acad Sci U S A
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Pruszak, J., Isacson, O.
Directed differentiation of human embryonic stem cells into dopaminergic neurons.
, (2007).
Pruszak, J., Sonntag, K. C., Aung, M. H., Sanchez-Pernaute, R., Isacson, O.
Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.
Stem Cells
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25
, 2257-2268 (2007).
Sonntag, K. C.
Enhanced yield of neuroepithelial precursors and midbrain-like dopaminergic neurons from human embryonic stem cells using the bone morphogenic protein antagonist noggin.
Stem Cells
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25
, 411-418 (2007).
Kawasaki, H.
Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity.
Proc Natl Acad Sci U S A
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99
, 1580-1585 (2002).
Ko, J. Y.
Human embryonic stem cell-derived neural precursors as a continuous, stable, and on-demand source for human dopamine neurons.
J Neurochem
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, 1417-1429 (2007).
Hong, S., Kang, U. J., Isacson, O., Kim, K. S.
Neural precursors derived from human embryonic stem cells maintain long-term proliferation without losing the potential to differentiate into all three neural lineages, including dopaminergic neurons.
J Neurochem
. , (2007).
Zhang, S. C.
Neural subtype specification from embryonic stem cells.
Brain Pathol
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16
, 132-142 (2006).
Pankratz, M. T.
Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
Stem Cells
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25
, 1511-1520 (2007).
Lazzari, G.
Direct derivation of neural rosettes from cloned bovine blastocysts: a model of early neurulation events and neural crest specification in vitro.
Stem Cells
.
24
, 2514-2521 (2006).
Pomp, O., Brokhman, I., Ben-Dor, I., Reubinoff, B., Goldstein, R. S.
Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells.
Stem Cells
.
23
, 923-930 (2005).
Tags
Human Embryonic Stem Cells
ES Cells
Propagation
Cell Culture
Growth
Maintenance
check_url/it/119?article_type=t
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DOI
Citazione di questo articolo
Daheron, L. Propagation of Human Embryonic Stem (ES) Cells.
J. Vis. Exp.
(1), e119, doi:10.3791/119 (2006).
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