Sample Preparation and Confocal Imaging of Dual-Colored F1 Transgenic Arabidopsis thaliana (Video) | JoVE
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Keywords:
Sample PreparationConfocal ImagingDual-colored F1 Transgenic Arabidopsis ThalianaTrue LeafAbaxialGlass SlideWaterCover SlipBeam Path514-nanometer LaserYellow Fluorescent Protein (YFP)PMTHYDEmission Band ThresholdsDetection Window530-570 Nanometers555-nanometer LaserRed Fluorescent Protein (RFP)570-630 Nanometers63X 1.2W Core LensSubcellular Membrane Traffic ActivityStomatal Precursor CellsImage SizeScan Head SpeedZoom FactorLine AverageXYT Scan ModeTime SeriesZ-stack Scan ModeMaximum Intensity ProjectionXYZ Scan ModeZ WideZ Step
Citazione di questo articolo
Sample Preparation and Confocal Imaging of Dual-Colored F1 Transgenic Arabidopsis thaliana. J. Vis. Exp. (Pending Publication), e200076, doi:10.3791/200076 (2023).