Infection of Cotyledons with <i>Agrobacterium rhizogenes</i> Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.

doi:10.3791/200165

JoVE Journal > Bioengineering

Bioingegneria

Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.

Published: May 05, 2023

doi:

10.3791/200165

Jie Lin, Dasol Wi, Melissa Ly, Md Asraful Jahan, Sarah Pullano, Izabella Martirosyan, Nik Kovinich

¹Department of Biology,York University, ²Department of Genetic Engineering and Biotechnology,Shahjalal University of Science and Technology

Tags

Agrobacterium Rhizogenes Soybean Cotyledons Hairy Roots TI Plasmid VirD2 PCR Spectinomycin Phosphate Buffer Acetosyringone Germination Medium Hairy Root Growth Elicitor Treatment Chemical Treatment RNA Extraction

Play Video

PDF

DOI

Citazione di questo articolo

Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.. J. Vis. Exp. (Pending Publication), e200165, doi:10.3791/200165 (2023).

Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.

Tags

Play Video

Citazione di questo articolo

View Video

✖

To prove you're not a robot, please enter the text in the image below