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Immunology and Infection

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Published: August 18, 2023

doi:

Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

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Keywords: Immunofluorescence Neutrophil Extracellular Traps Blocking Solution Donkey Serum Primary Antibodies Antibody Dilution Buffer Myeloperoxidase Citrullinated Histone H3 Neutrophil Elastase Secondary Antibodies DAPI Mounting Medium

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Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples. J. Vis. Exp. (Pending Publication), e200337, doi:10.3791/200337 (2023).

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