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Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens (Video) | JoVE

Published: August 25, 2023

doi:

Brittany Turner-Ivey, Jody Fromm Longo, Dorea P. Jenkins, Stephen T. Guest, Steven L. Carroll²

¹Hollings Cancer Center,Medical University of South Carolina, ²Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ³Department of Computational Medicine and Bioinformatics,University of Michigan, Ann Arbor

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Lentiviral Packaging Viral Titering Genome-scale ShRNA Screens 293T Cells Packaging Plasmid Mix Plasmid Barcode Library Transfection Reagent Viral Particles Viral Titering Cationic Polymer Tumor Cell Growth Media Tumor Cells Lentivirus

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DOI

Citazione di questo articolo

Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens. J. Vis. Exp. (Pending Publication), e200477, doi:10.3791/200477 (2023).

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