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Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster (Video) | JoVE

Published: November 10, 2023
doi:
10.3791/200718
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1Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences,Federal University of Santa Maria, 2Graduate Program in Biological Sciences: Toxicological Biochemistry,Federal University of Santa Maria

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Keywords: Mitochondrial RespirationDrosophila MelanogasterSubstrateUncouplerInhibitorTitrationPolarographic Oxygen SensorsMiR05 BufferHigh-resolution RespirometryDigitoninPyruvateMalateProlineADPSuccinateOligomycinFCCPRotenoneMalonateAntimycinOXPHOS CIOXPHOS CIIETS CIETS CIIATP SynthesisOXPHOS/LEAK Ratio
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Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster. J. Vis. Exp. (Pending Publication), e200718, doi:10.3791/200718 (2023).
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