High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity

Published: December 01, 2023

doi:

10.3791/201076

Adrienne Goppold, Louis Conradie, Otávio Guilherme Gonçalves de Almeida, Elaine Cristina Pereira De Martinis, Folarin Anthony Oguntoyinbo

¹A.R. Smith Department of Chemistry and Fermentation Sciences,Appalachian State University, ²Faculdade de Ciências Farmacêuticas de Ribeirão Preto,Universidade de São Paulo

Tags

Keywords: 16S RRNA Gene V4 Hypervariable Region PCR Sequencing Adapter Ligation Magnetic Beads DNA Concentration Fluorimeter FASTQ Files Mapping File QIIME2 DADA2 Naive Bayes Classifier Silva Database OTU Taxonomic Assignment MicrobiomeDB

Play Video

PDF

DOI

Citazione di questo articolo

High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity. J. Vis. Exp. (Pending Publication), e201076, doi:10.3791/201076 (2023).

High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity

Tags

Play Video

Citazione di questo articolo

View Video

✖

To prove you're not a robot, please enter the text in the image below