Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

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Biologia

Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

Published: October 06, 2023

doi:

10.3791/201250

Humna Hasan, Andrea L. Kasinski²

¹Department of Biological Sciences,Purdue University, ²Purdue Institute for Cancer Research,Purdue University

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Keywords: Flow Cytometry Extracellular Vesicles EV-miRNA Cell-miRNA Fluorophore-conjugated MiRNA Calu6 Cells Nano-flow Cytometry Performance Beads Instrument Calibration Gating Sample Preparation Data Analysis

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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA. J. Vis. Exp. (Pending Publication), e201250, doi:10.3791/201250 (2023).

Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

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