Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation - JoVE

JoVE Journal > Biochemistry

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Published: January 26, 2024

doi:

Shreenidhi Rajkumar, Danielle Dixon, Andrew M. Lipchik, Joshua J. Gruber

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Tags

Keywords: Click Assay Histone Acetyltransferase 1 Acetylation Biotinylated Histone H4 Peptide Acetylation Reactions 4-Penten Oil Co-enzyme A BSA Pre-blocked Black NeutrAvidin-coated 96-well Plates THPTA Copper Mixture Sodium Ascorbate Biotin Azide Streptavidin Horseradish Peroxidase Amplex Red Detection Reagents Hydrogen Peroxide Sodium Phosphate

Play Video

DOI

Citazione di questo articolo

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation. J. Vis. Exp. (Pending Publication), e201342, doi:10.3791/201342 (2024).

View Video