Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies

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Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies

Published: February 09, 2024

doi:

10.3791/201469

Kathryn K. Harris, Oleksandr Kondratov, Sergei Zolotukhin

¹Department of Pediatrics,University of Florida

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Keywords: Adeno-associated Virus AAV Iodixanol Ultracentrifugation Purification In Vivo Studies Virus Clarification Density Gradient Centrifugation Peristaltic Pump Virus Fraction 15% Iodixanol 25% Iodixanol 40% Iodixanol 60% Iodixanol Needle Aspiration Virus Dilution Lysis Buffer

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Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies. J. Vis. Exp. (Pending Publication), e201469, doi:10.3791/201469 (2024).

Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies

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