Source: Green, N. H. et al. Production, characterization and potential uses of a 3D tissue-engineered human esophageal mucosal model. J. Vis. Exp. (2015)
In this video, we demonstrate the preparation of a decellularized pig esophagus scaffold, with its biochemical architecture and composition preserved, for tissue engineering applications.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Decellularized Esophageal Scaffold
The authors have nothing to disclose.
DMEM | Labtech | LM-D1112 | Warm in 37 °C water bath before use |
Ham's F12 | Labtech | LM-H1236 | Warm in 37 °C water bath before use |
Foetal Calf Serum | Labtech | FB-1090 | |
Epidermal Growth Factor | R+D Systems | 236-EG-200 | Prepare 200 µg/ml stock solution in 10 mM acetic acid, 1% FCS |
Cholera toxin | Sigma-Aldrich | C8052 | Prepare stock solution in water |
L-Glutamine | Sigma-Aldrich | G7513 | |
Penicillin-Streptomycin | Sigma-Aldrich | P0781 | |
Amphotericin B | Gibco | 15290-026 | Brand name Fungizone |
PBS | Oxoid | BR0014 | Dissolve 1 tablet in 100 ml water and autoclave to sterilize |
Collagenase A | Roche | 10103578001 | |
Povidone-iodine solution | Ecolab | 10830E | Brand name Videne |
Ethanol | Sigma-Aldrich | E7023 | |
Chelex 100 | Sigma-Aldrich | C7901 | |
Newborn calf serum | Gibco | 26010074 | |
15 ml universal tube | SLS | SLS7504 | |
180 ml pot | VWR | 216-2603 |