Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c–>C57BL/6 skin transplant.
Prior to the initiation of experiment:
I. Harvest Donor Tissue
II. Prepare Recipient for Transplant
III. Skin Transplant
IV. Bandage Removal (6-7 days post-transplant)
V. Helpful Hints
As demonstrated in this video-article, the skin transplant model is a quick and easy method for monitoring allogeneic T cell responses. Allogeneic skin transplantation has a wide range of applications and has been utilized to assay the efficiency of a variety of immunosuppressive agents and tolerance induction protocols, as well as to investigate the biological effects of various blocking antibodies.
The authors have nothing to disclose.
NIH Immunology Training Grant T32 AI 60573 (KRG) and NIH grant GM 41514 (MDC)
Material Name | Tipo | Company | Catalogue Number | Comment |
---|---|---|---|---|
Micro-dissecting forceps (curved) | Sigma | F4142 | ||
Rat-tooth forceps (1×2 teeth, straight) | Fine Science Tools | 11053-10 | n=2 | |
Strabismus scissors (blunt end) | Fine Science Tools | 14074-11 | ||
Iris forceps (curved) | Fine Science Tools | 11065-07 | ||
Spring scissors (angled) | Fine Science Tools | 15006-09 | ||
Halsey micro needle holder | Fine Science Tools | 12500-12 | ||
10 cm plates | Corning | 430167 | ||
PBS | Cellgro | 21-040-CV | ||
Bupernex (Buprenorphine hydrochloride) | Reckitt Bencikiser Healthcare | 517201 | ||
Insulin syringes (29 Ga) | Monoject | 8881 600145 | ||
Q-tips | Solon | 362 | ||
Single edge razor blades (#9) | Smith | 67-0238 | ||
Silk suture (2-0; PS-2) | Ethicon | 583H |