Counting and Determining the Viability of Cultured Cells
Summary
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
Abstract
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.
Protocol
The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
Divulgazioni
The authors have nothing to disclose.
Citazione di questo articolo
Ricardo, R., Phelan, K. Counting and Determining the Viability of Cultured Cells. J. Vis. Exp. (16), e752, doi:10.3791/752 (2008).