Adipose Tissue-Derived Stem Cell Isolation and Characterization

April 07, 2023

Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Murine Adipose Tissue Excision

Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization

Immunofluorescent Detection of Adipose-Derived Stem Cell Expression Markers

Flow Cytometric Detection of Adipose-Derived Stem Cell Expression Markers

Adipose-Derived Stem Cell Differentiation to the Adipogenic Lineage

Aymé Oliva Cárdenas², Blanca C. Zamora-Rodríguez, Kevin A. Batalla-García, Alejandro Ávalos-Rodríguez, Alejandra Contreras-Ramos, Clara Ortega-Camarillo

¹Doctorado en Ciencias Biológicas y de la Salud,Universidad Autónoma Metropolitana, ²Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI,Instituto Mexicano de Seguro Social, ³Department of Agricultural and Animal Production, Division of Biological and Health Science,Universidad Autónoma Metropolitana-Xochimilco, ⁴Molecular Biology Research Laboratory, Center for Congenital Malformations,Children Hospital of Mexico Federico Gomez (HIMFG)

Summary

This protocol describes a methodology for isolating and identifying adipose tissue-derived mesenchymal stem cells (MSCs) from Sprague Dawley rats.

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