Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

Ana Filipa Isidro; Alexandra M. Medeiros; Isaura Martins; Dalila Neves-Silva; Leonor Sa&#250;de; C&#233;sar S. Mendes

doi:10.3791/65207

Quantifying Locomotor Dysfunction in Spinal Cord Injury Mice Using MouseWalker

March 24, 2023

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury (Video) | JoVE

02:40 min

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Setting Up of the MouseWalker (MW) Apparatus (Video) | JoVE

01:08 min

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Video Acquisition and Preparing the Videos for the MouseWalker (MW) Tracking Software (Video) | JoVE

01:10 min

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MouseWalker (MW) Video Tracking and Kinematic Data Analysis (Video) | JoVE

03:07 min

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Ana Filipa Isidro, Alexandra M. Medeiros, Isaura Martins, Dalila Neves-Silva, Leonor Saúde³, César S. Mendes

¹Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa, ²iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, NMS|FCM,Universidade Nova de Lisboa, ³Instituto de Histologia e Biologia do Desenvolvimento,Faculdade de Medicina da Universidade de Lisboa

Summary

An experimental pipeline to quantitatively describe the locomotor pattern of freely walking mice using the MouseWalker (MW) toolbox is provided, ranging from initial video recordings and tracking to post-quantification analysis. A spinal cord contusion injury model in mice is employed to demonstrate the usefulness of the MW system.