Confocal Microscopic Analysis of Rat Muscle Mitochondria

December 01, 2023

Mitochondrial Remodeling in Skeletal Muscle (Video) | JoVE

01:43 min

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Rat Gastrocnemius Muscle Fiber Bundle Dissection (Video) | JoVE

01:06 min

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Confocal Live Cell Imaging of Rat Skeletal Muscle Mitochondria (Video) | JoVE

03:04 min

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Mitochondrial Density Analysis (Video) | JoVE

02:28 min

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Mitochondrial Distribution Analysis Using Fast Fourier Transform (Video) | JoVE

02:47 min

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Perla Pérez-Treviño, Bianca Nieblas², Selma Romina López-Vaquera, Noemí García^2,3

¹Experimental Medicine and Advanced Therapies, The Institute for Obesity Research,Tecnologico de Monterrey, ²Escuela de Medicina y Ciencias de la Salud,Tecnologico de Monterrey, ³Preclinical Research Unit,Tecnologico de Monterrey

Summary

Here, we present a protocol to analyze changes in mitochondrial density and longitudinal distribution by live-skeletal muscle imaging using confocal microscopy for mitochondrial network scanning.