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Embryonic Mouse Brain Mixed Cell Cultures to Study Infection and Innate Immunity

June 30, 2023
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Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity (Video) | JoVE

01:46 min
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Murine E17 Embryonal Brain Dissection, Cell Plating, and Maintenance (Video) | JoVE

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1School of Infection and Immunity,University of Glasgow, 2Faculty of Science and Technology, Institute of Technology,University of Tartu

Summary

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This protocol presents a unique way of generating central nervous system cell cultures from embryonic day 17 mouse brains for neuro(immuno)logy research. This model can be analyzed using various experimental techniques, including RT-qPCR, microscopy, ELISA, and flow cytometry.

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