Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

July 07, 2023

Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study (Video) | JoVE

01:27 min

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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing (Video) | JoVE

05:45 min

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Szu-Ying Chen², Po-Hsien Huang²

¹Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University

Summary

This protocol provides an easy-to-follow workflow to conduct poly(A) RNA purification, bisulfite conversion, and library preparation using standardized equipment for a biological sample of interest.

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study (Video) | JoVE

Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing (Video) | JoVE

Summary

Article