Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Ayona Gupta; Risani Mukhopadhyay; Himanshi Khandelwal; Narendra Nala; Uttara Chakraborty

doi:10.3791/65723

Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

November 10, 2023

Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells (Video) | JoVE

02:14 min

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A Step-by-Step Guide for Seeding and Differentiation of Mesenchymal Stem Cell Cultures for Characterization (Video) | JoVE

02:13 min

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Lineage-Specific Cytochemical Staining of Mesenchymal Stem Cells for Characterizing Differentiated Cells (Video) | JoVE

02:06 min

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Mesenchymal Stem Cell Surface Staining for Immunophenotyping (Video) | JoVE

02:33 min

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Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting (Video) | JoVE

05:25 min

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Ayona Gupta*¹, Risani Mukhopadhyay*¹, Himanshi Khandelwal, Narendra Nala, Uttara Chakraborty

¹Manipal Institute of Regenerative Medicine, Bengaluru,Manipal Academy of Higher Education, Manipal, ²HIV-AIDS Laboratory, Molecular Biology and Genetics Unit,Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

Summary

This protocol describes the use of fluorescence-activated cell sorting of human mesenchymal stem cells using the single-cell sorting method. Specifically, the use of single-cell sorting can achieve 99% purity of the immunophenotyped cells from a heterogeneous population when combined with a multiparametric flow cytometry-based approach.