Investigating Development with Optogenetic Signaling Manipulation in Zebrafish

October 27, 2023

斑马鱼胚胎中的光遗传学信号激活

将 bOpto-BMP/Nodal 构建体注射到斑马鱼胚胎中

激活斑马鱼BMP或淋巴结信号的光照实验

光照实验后bOpto-BMP/Nodal表达胚胎的评价

Allison J. Saul*¹, Catherine E. Rogers*¹, Marcial Garmendia-Cedillos, Thomas Pohida, Katherine W. Rogers

¹Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NIH, ²Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering,NIH

Summary

信号通路的光遗传学操作可以成为研究信号转导在发育、再生、稳态和疾病中如何解码的有力策略。该协议为在早期斑马鱼胚胎中使用基于光 – 氧 – 电压传感域的节点和骨形态发生蛋白（BMP）信号激活剂提供了实用指南。

Article