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Visualization of Cellular Interactions Between the Maize Sheaths and Fungi

September 15, 2023
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Investigating Fungal Pathogenicity Mechanisms in Maize (Video) | JoVE

00:49 min
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Inoculation of Maize Leaf Sheaths with Pathogenic Fungi for Assessing Fungal Pathogenicity (Video) | JoVE

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Preparation of Fungal Infected Detached Maize Leaf Sheaths for Live-Cell Imaging (Video) | JoVE

02:06 min
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, , , , ,
1Department of Plant Pathology,University of Kentucky, 2Department of Biological Sciences,University of Cincinnati, 3Bayer Crop Science, 4Everglades Research and Education Center,University of Florida

Summary

Traduzione automatica

This manuscript details an optimized inoculation protocol that uses detached maize leaf sheaths for reproducible cytological, physiological, and molecular studies of maize interactions with fungal plant pathogens. The leaf sheaths facilitate real-time observation of cellular interactions between the living plant and fungus in unfixed tissues.

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