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Quantifying Vaccine-Induced Neutralizing Antibodies in SARS-CoV-2 Variants

September 08, 2023
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Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies

01:18 min
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Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

01:40 min
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Quantification of Ha-CoV-2 Variants and 27BV Neutralization Activity Against Ha-CoV-2(Luc) Variants

03:13 min
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1Center for Infectious Disease Research, School of Systems Biology,George Mason University

Summary

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This protocol describes the application of a novel hybrid alphavirus-SARS-CoV-2 pseudovirus (Ha-CoV-2) as a platform for rapid quantification of infectivity of SARS-CoV-2 variants and their sensitivity to neutralizing antibodies.

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