1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research,The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute,Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care,Oregon Health & Science University, 5Department of Surgery,Oregon Health & Science University, 6Department of Molecular & Medical Genetics,Oregon Health & Science University
Il presente protocollo descrive la riprogrammazione dell’adenocarcinoma duttale pancreatico (PDAC) e delle cellule epiteliali duttali pancreatiche normali in cellule staminali pluripotenti indotte (iPSC). Forniamo una procedura ottimizzata e dettagliata, passo dopo passo, dalla preparazione dei lentivirus alla creazione di linee iPSC stabili.
