1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research,The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute,Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care,Oregon Health & Science University, 5Department of Surgery,Oregon Health & Science University, 6Department of Molecular & Medical Genetics,Oregon Health & Science University
O presente protocolo descreve a reprogramação do Adenocarcinoma Ductal Pancreático (ADP) e de células epiteliais ductais pancreáticas normais em células-tronco pluripotentes induzidas (iPSCs). Fornecemos um procedimento otimizado e detalhado, passo a passo, desde a preparação do lentivírus até o estabelecimento de linhas iPSC estáveis.
