T4 Bacteriophage and <i>E. coli</i> Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo

Nicola Pett; Michael Hunter; Natalia A. Carranza Garc&#237;a; Jung Hee Seo; Samuel R. Collins; Forest Rohwer; Lisa C. Osborne; Carolina Tropini

doi:10.3791/65906

Exploring Gut Phage-Bacteria Dynamics in Murine Models Using T4 Phage

January 26, 2024

T4 Bakteriofag og E. coli-interaktion i murintarmen: En prototypisk model til undersøgelse af værtsbakteriofagdynamik in vivo

00:56 min

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Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

05:18 min

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In Vivo Monitoring and Quantification of T4 Phage in Mice Using Fecal Spot Plating Assays

02:40 min

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Nicola Pett, Michael Hunter, Natalia A. Carranza García, Jung Hee Seo, Samuel R. Collins, Forest Rohwer, Lisa C. Osborne, Carolina Tropini^3,4

¹Department of Microbiology and Immunology, Life Sciences Institute,University of British Columbia, ²Department of Biology,San Diego State University, ³School of Biomedical Engineering,University of British Columbia, ⁴Humans and the Microbiome Program,Canadian Institute for Advanced Research

Summary

Bakteriofager (fager), vira, der inficerer bakterier, er en integreret bestanddel af tarmmikrobiomet. Selvom disse symbiotiske indbyggere driver bakteriel fitness og befolkningsdynamik, forstås der lidt om, hvordan de påvirker tarmhomeostase og sygdom. Denne protokol studerer isolerede T4-fager inden for en musemodel, der kan tilpasses andre fagbakteriepar.

Exploring Gut Phage-Bacteria Dynamics in Murine Models Using T4 Phage

T4 Bakteriofag og E. coli-interaktion i murintarmen: En prototypisk model til undersøgelse af værtsbakteriofagdynamik in vivo

Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

In Vivo Monitoring and Quantification of T4 Phage in Mice Using Fecal Spot Plating Assays

Summary

Article