Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

Roy Cohen; Danielle M. Sosnicki; Melissa A. White; Jacquelyn L. Nelson; Chinatsu Mukai; Alexander J. Travis

doi:10.3791/65962

Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13, 2023

A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm (Video) | JoVE

00:46 min

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Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging (Video) | JoVE

01:27 min

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Setting up the Single Cell Stimulant Delivery System for Real-Time Monitoring of Live Mouse Sperm (Video) | JoVE

01:19 min

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Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse (Video) | JoVE

01:45 min

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Roy Cohen², Danielle M. Sosnicki, Melissa A. White, Jacquelyn L. Nelson, Chinatsu Mukai, Alexander J. Travis²

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Summary

The AcroSensE mouse model and live cell imaging methods described here provide a new approach to studying calcium dynamics in the subcellular compartment of the sperm acrosome and how they regulate intermediate steps leading to membrane fusion and acrosome exocytosis.