Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13, 2023

Real-time imaging av akrosomal kalsiumdynamikk og eksocytose i levende mus sperm

00:46 min

•

Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging

01:27 min

•

Setting up the Single Cell Stimulant Delivery System for Real-Time Monitoring of Live Mouse Sperm

01:19 min

•

Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse

01:45 min

•

Roy Cohen², Danielle M. Sosnicki, Melissa A. White, Jacquelyn L. Nelson, Chinatsu Mukai, Alexander J. Travis²

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Summary

AcroSensE-musemodellen og levende celleavbildningsmetoder beskrevet her, gir en ny tilnærming til å studere kalsiumdynamikk i det subcellulære rommet til sædakrosomet og hvordan de regulerer mellomliggende trinn som fører til membranfusjon og akrosomeksocytose.