Fluorescent Labeling of Glomeruli in Cleared Mouse Kidneys for Microscopy

February 09, 2024

Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues (Video) | JoVE

01:36 min

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Tissue Clearing of Mouse Kidneys for Confocal Microscopy (Video) | JoVE

01:25 min

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Confocal and Light Sheet Fluorescence Microscopy (LSFM) Imaging of Cleared Mouse Kidneys (Video) | JoVE

04:10 min

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Data Processing and Quantification of Glomeruli from Microscopy Images (Video) | JoVE

02:52 min

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Lin Bai², Yaping Wu, Wenshu Dai, Qiuxiao Shi, Lei Wu, Jie Zhang², Lily Zheng

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

Summary

This study presents an easy-to-use, complete, and simple set of methods to label and analyze glomeruli from CUBIC-cleared mouse kidneys. Data such as glomerulus number and volume can be obtained easily and reliably using fluorescein isothiocyanate (FITC)-Dextran, light sheet fluorescence microscopy (LSFM), or common confocal microscopy and software such as Imaris.