An <em>Ex Vivo</em> Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum

Terrence Wong; Roslyn Tedja; Hussein Chehade; Robert Morris; Ayesha B. Alvero; Gil Mor

doi:10.3791/66031

Establishing a 3D Ex Vivo Model for Ovarian Cancer-Omentum Interaction

January 26, 2024

Advanced Ex Vivo Model for Investigating Cancer-Adipose Microenvironment Interaction (Video) | JoVE

01:38 min

•

Human Omentum Specimen Processing and Co-Culture with Ovarian Cancer Cells (Video) | JoVE

04:24 min

•

Terrence Wong, Roslyn Tedja, Hussein Chehade, Robert Morris, Ayesha B. Alvero, Gil Mor

¹Department of Gynecologic Oncology,Karmanos Cancer Institute, ²C.S. Mott Center for Human Growth and Development, Department of Obstetrics and Gynecology,Wayne State University School of Medicine

Summary

This protocol describes the establishment of a three-dimensional (3D) ex vivo model of cancer cell-omentum interaction. The model provides a platform for elucidating pro-tumor mechanisms within the adipose niche and for testing novel therapies.

Establishing a 3D Ex Vivo Model for Ovarian Cancer-Omentum Interaction

Advanced Ex Vivo Model for Investigating Cancer-Adipose Microenvironment Interaction (Video) | JoVE

Human Omentum Specimen Processing and Co-Culture with Ovarian Cancer Cells (Video) | JoVE

Summary

Article