Live Cell Single-Molecule Imaging-Based Quantification of Protein Interactions

January 05, 2024

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

00:43 min

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Halo-Ligand Staining of Cells for Protein Condensate-Interaction Studies Using Single-Molecule Imaging

00:53 min

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Single-Molecule Imaging of Halo-Tagged Proteins to Study Protein-Condensate Interactions

01:56 min

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Analysis of Single-Molecule Imaging Data to Study Interactions of Halo-Tagged Proteins

03:22 min

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Shawn R. Yoshida², Shasha Chong

¹Division of Chemistry and Chemical Engineering,California Institute of Technology, ²Division of Biology and Biological Engineering,California Institute of Technology

Summary

Many intrinsically disordered proteins have been shown to participate in the formation of highly dynamic biomolecular condensates, a behavior important for numerous cellular processes. Here, we present a single-molecule imaging-based method for quantifying the dynamics by which proteins interact with each other in biomolecular condensates in live cells.