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Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

April 5th, 2024
*1,2,3, *1,2,3, 1,2,4, 1,2,5, 1,2,3, 1,2,3, 1,2,3,6, *1,2,4,5, *1,2,3,4
1CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, 2CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3IIIUC - Institute for Interdisciplinary Research, University of Coimbra, 4ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, 5FFUC - Faculty of Pharmacy, University of Coimbra, 6MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra
* These authors contributed equally

Summary
Automatic Translation

This manuscript describes a detailed protocol for the generation and purification of adeno-associated viral vectors using an optimized heparin-based affinity chromatography method. It presents a simple, scalable, and cost-effective approach, eliminating the need for ultracentrifugation. The resulting vectors exhibit high purity and biological activity, proving their value in preclinical studies.

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