Validating Xanthatin-Keap1 Interaction with Docking and CETSA

February 23, 2024

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

01:06 min

•

Molecular Docking to Investigate the Interaction Between Xanthatin and Keap1 Protein

02:37 min

•

Cell Culture and Cellular Thermal Shift Assay (CETSA) Sample Preparation for Western Blotting

02:35 min

•

Verification of the Interaction Between Xanthatin and Keap1 Protein Using Western Blotting

04:10 min

•

Liuling Luo, Jinrui Xiong, Yancai Tang, Xiaorui Chen, Hai Zhang

¹State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine

Summary

The experiment used here shows a method of molecular docking combined with cellular thermal shift assay to predict and validate the interaction between small molecules and protein targets.