Sigma-Aldrich View Institution's Website 8 articles published in JoVE Application Notes LookOut Mycoplasma Elimination Kit - ADVERTISEMENT Paige L. Dobrovolny1 1Product Management, Sigma-Aldrich The LookOut Mycoplasma elimination kit combines biological agents that reliably and completely eliminate mycoplasma contamination with minimal cytotoxic effect on cells. Biology Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs) Keith Hansen1, Matthew J. Coussens1, Jack Sago1, Shilpi Subramanian1, Monika Gjoka1, Dave Briner1 1Emerging Technologies, Sigma Life Science The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service. Biology Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library Matthew J. Coussens*1, Kevin Forbes*1, Carol Kreader*1, Jack Sago1, Carrie Cupp1, John Swarthout1 1Sigma-Aldrich The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application. Biology MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells Matthew J. Coussens1, Courtney Corman1, Ashley L. Fischer1, Jack Sago1, John Swarthout1 1Sigma-Aldrich Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results. Biology Optimized PCR-based Detection of Mycoplasma Paige L. Dobrovolny1, Dan Bess1 1Product Management, Sigma-Aldrich The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals. Biology Use of the Protease Fluorescent Detection Kit to Determine Protease Activity Carrie Cupp-Enyard1 1Sigma Aldrich The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate. Application Notes Sigma公司的以酪蛋白为底物泛蛋白酶活性检测方法 - ADVERTISEMENT Carrie Cupp-Enyard1 1Sigma Aldrich Biology Sigma's Non-specific Protease Activity Assay - Casein as a Substrate Carrie Cupp-Enyard1 1Sigma Aldrich Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases.