Capital Medical University 24 articles published in JoVE Medicine Examination of Oral Candida Infection in Primary Sjögren's Syndrome Patients Xiaoyan Wang1,2,3, Yeqing Song4, Xinzhe Lou1, Bingqing Han5,6,7, Ruiqing Wu1, Jing Xie8,9, Xiang Lin8,9, Hao Wang1 1Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, 2Beijing Laboratory of Oral Health, Capital Medical University, 3Department of Biochemistry and Molecular Biology, School of Basic Medicine, Capital Medical University, 4Central Laboratory, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, 5Clinical Diagnosis Laboratory of Beijing Tiantan Hospital, Capital Medical University, 6Key Laboratory for Quality Control of In Vitro Diagnostics, NMPA, 7Beijing Engineering Research Center of Immunological Reagents Clinical Research, 8School of Chinese Medicine, The University of Hong Kong, 9Department of Rheumatology and Clinical Immunology, Beijing Tiantan Hospital, Capital Medical University Here, we present a protocol for the examination of oral Candida infection in patients with primary Sjögren's syndrome, which can be used for timely treatment and, thereafter, avoiding related complications. Medicine Three-Dimensional Reconstruction for the Whole Lung with Early Multiple Pulmonary Nodules Junyi Shi1, Fangliang Xing2, Yang Liu3, Tengxiao Liang4 1Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 4Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine This study introduces a three-dimensional (3D) reconstruction method for the entire lung in patients with early multiple pulmonary nodules. It offers a comprehensive visualization of nodule distribution and their interplay with lung tissue, simplifying the assessment of diagnosis and prognosis for these patients. Medicine A Three-Dimensional Digital Model for Early Diagnosis of Hepatic Fibrosis Based on Magnetic Resonance Elastography Yang Liu1, Tengxiao Liang2, Fangliang Xing3, Wei Hou4, Xiaofei Shang1, Xiuhui Li1 1Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 2Fever Clinics, Dongzhimen Hospital of Beijing University of Chinese Medicine, 3Beijing Intelligent Entropy Science& Technology Co Ltd., 4First Department of The Liver Disease Center, Beijing You An Hospital, Capital Medical University The objective of this study was to develop a novel three-dimensional digital model for the early diagnosis of hepatic fibrosis, which includes the stiffness of each voxel in the patient's liver and can thus, be used to calculate the distribution ratio of the patient's liver at different fibrosis stages. Neuroscience A Procedure for Mouse Dorsal Root Ganglion Cryosectioning Liangliang He*1,2, Wenxing Zhao*1, Lingyi Zhang2,3, Maalveka Ilango2,4, Na Zhao2, Liqiang Yang1, Zhonghui Guan2 1Department of Pain Management, Xuanwu Hospital, Capital Medical University, 2Department of Anesthesia and Perioperative Care, University of California San Francisco, 3Department of Anesthesiology, Sun Yat-Sen University, 4University of Washington Presented here is the development for consistently acquiring high-quality dorsal root ganglion cryostat sections. Medicine A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules Jingwen Xue1, Fangliang Xing2, Yang Liu3, Tengxiao Liang4 1Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 4Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine The objective of this study is to develop a novel 3D digital model of pulmonary nodules that serves as a communication bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic evaluation. Cancer Research Surgical Technique for Superior Cervical Ganglionectomy in a Murine Model Qi Wang1, Chun-Hao Chen1, Hongbo Xu2, Sylvie Deborde1,3, Richard J. Wong1,3 1Department of Surgery, Memorial Sloan Kettering Cancer Center, 2Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 3David M. Rubenstein Center for Pancreatic Cancer Research, Memorial Sloan Kettering Cancer Center The present protocol describes a mouse model of the ablation of adrenergic innervation by identifying and resecting the superior cervical ganglion. Cancer Research Reconstruct Human Retinoblastoma In Vitro Xiao Zhang1, Zi-Bing Jin1 1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish. Medicine Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice Kui Wu1, Lin-Ling Li2, Yu-Kun Li1, Xiao-Dong Peng1, Meng-Xia Zhang1, Ke-Sen Liu1, Xue-Si Wang1, Jia-Xue Yang1, Song-Nan Wen1, Yan-Fei Ruan1, Nian Liu1, Rong Bai1 1Department of Cardiology, Bejing Anzhen Hospital, Capital Medical University, 2Department of Cardiology, Bejing Chuiyangliu Hospital This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice. Medicine Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells Xiao Zhang1, Zi-Bing Jin1 1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory Using a self-organizing method, we develop a protocol with the addition of COCO that could significantly increase the generation of photoreceptors. Neuroscience Detrusor Underactivity Model in Rats by Conus Medullaris Transection Xin Zheng*1, Menghua Wu*1, Jian Song2, Jimao Zhao2 1Department of Urology, Beijing YouAn Hospital, Capital Medical University, 2Department of Urology, Beijing Friendship Hospital, Capital Medical University We present a method for establishing a detrusor underactivity model by conus medullaris transection in rats. Detrusor underactivity was successfully stimulated in these animals. The model can be used for studying urinary tract function. Medicine Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography Di Liu*1, Xizhu Xu*2, Yuejin Li2, Jie Zhang1, Xiaoyu Zhang1, Qihuan Li1, Haifeng Hou2, Dong Li2, Wei Wang1,2,3, Youxin Wang1 1Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, 2School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, 3School of Medical and Health Sciences, Edith Cowan University Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings. Medicine Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP) PeiLun Hu*1, JiSheng Lin*1, JunChuan Xu1, Hai Meng1, Nan Su1, Yong Yang*1, Qi Fei1 1Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case study. Neuroscience Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure Honghui Mao*1,2, Xiuchao Wang*1,3, Wen Chen4, FengYu Liu5, You Wan5, Sanjue Hu1, Junling Xing1,6 1Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, 2Department of Toxicology, School of Public Health, ShanXi Medical University, 3Department of Psychology, Fourth Military Medical University, 4Department of Neurobiology, School of Basic Medical Sciences, Advanced Innovation Center for Human Brain Protection, Capital Medical University, 5Neuroscience Research Institute, Key Lab for Neuroscience, Ministry of Education/National Health Commission, Peking University, 6Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University Single-fiber recording is an effective electrophysiological technique that is applicable to the central and peripheral nervous systems. Along with the preparation of intact DRG with the attached sciatic nerve, the mechanism of conduction failure is examined. Both protocols improve the understanding of the peripheral nervous system's relationship with pain. Neuroscience Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea Shu-Kui Yu*1, Zheng-De Du*1, Qing-Ling Song1, Teng-Fei Qu1, Yue Qi1, Wei Xiong1, Lu He1, Wei Wei2, Shu-Sheng Gong1, Ke Liu1 1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, 2Department of Otology, Shengjing Hospital of China Medical University This manuscript describes an experimental protocol for evaluating the morphological characteristics and functional status of ribbon synapses in normal mice. The present model is also suitable for noise-induced and age-related cochlear synaptopathy-restricted models. The correlative results of previous mouse studies are also discussed. Medicine A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica Wen Cao1, Xiaoli Zhu2, Ziren Tang3, Yuguo Song2 1Department of Ultrasound Medicine, Beijing Chaoyang Hospital, Capital Medical University, 2Department of Occupational Medicine & Clinical Toxicology, Beijing Chaoyang Hospital, Capital Medical University, 3Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University Here, we present a protocol to construct a pleural effusion model in rats by intratracheal instillation of polyacrylate/nanosilica. Environment A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans Shan Gao*1, Weiyang Chen*2, Nan Zhang1, Chi Xu3, Haiming Jing1,4, Wenjing Zhang1,4, Gaochao Han1,4, Matthew Flavel5, Markandeya Jois5, Yingxin Zeng1, Jing-Dong J. Han3, Bo Xian3, Guojun Li1,4 1Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, China, 2College of Computer Science and Technology, Qilu University of Technology(Shandong Academy of Sciences), China, 3Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Collaborative Innovation Center for Genetics and Developmental Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China, 4Beijing Key Laboratory of Environmental Toxicology, School of Public Health, Capital Medical University, China, 5School of Life Sciences, La Trobe University, Australia A quantitative method has been developed to identify and predict the acute toxicity of chemicals by automatically analyzing the phenotypic profiling of Caenorhabditis elegans. This protocol describes how to treat worms with chemicals in a 384-well plate, capture videos, and quantify toxicological related phenotypes. Cancer Research Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad Gan-Lin Zhang1, Yi Zhang1, Ke-Xin Cao1, Xiao-Min Wang1 1Oncology Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University Here, we present a protocol to implant breast cancer cells into the mammary fat pad in a simple, less invasive, and easy-to-handle way, and this mouse orthotopic breast cancer model with a proper mammary fat pad environment can be used to investigate various aspects of cancer. Engineering Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro Zilan Xiong*1, Shasha Zhao*2, Xu Yan3 1State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, 2College of Life Science and Health, Wuhan University of Science and Technology, 3Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement. Genetics Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice Jing-Ying Guo1, Lu He1, Teng-Fei Qu1, Yu-Ying Liu2, Ke Liu1, Guo-Peng Wang1, Shu-Sheng Gong1 1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, 2Department of Otolaryngology-Head and Neck Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University Here we describe canalostomy procedure which allows local drug delivery into the inner ears of adult and neonatal mice through the semicircular canal with minimum damage to hearing and vestibular function. This method can be used to inoculate viral vectors, pharmaceuticals, and small molecules into the mouse inner ear. Immunology and Infection An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection Chongfeng Xu1, Junhong Ai2, Qingxun Zhang1, Ting Li3, Xiaorong Wu1, Zhengde Xie2, Ziyuan Duan1 1Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, 3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences A simple method for obtaining NK and T cell clones from CAEBV patients was developed with high efficiency, a small amount of peripheral blood, and a low-dose of IL-2. Developmental Biology Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis Erica L. McGrath1,2, Junling Gao1, Ping Wu1,3 1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Neurology, Johns Hopkins University, 3Beijing Institute for Brain Disorders, Capital Medical University This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection. Neuroscience Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells Fancheng Yan1, Sailiang Guo2, Yijie Chai2, Lan Zhang3, Kegao Liu3, Qingjun Lu4, Ningli Wang4, Shuning Li3 1Aier School of Ophthalmology, Central South University, Changsha, China, 2Institute of Immunology, Tsinghua University School of Medicine, Beijing, China, 3Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China, 4Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China Secondary degeneration of retinal ganglion cells (RGCs) occurs commonly in glaucoma. This study describes an innovative operative approach for partial optic nerve transection. The use of this space-saving operative approach extends the model's application range, and allows exploration of secondary injury mechanisms in RGCs in a new way. Engineering New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells Xu Yan*1,3,4,5, Zhaozhong Meng*2, Jiting Ouyang2, Yajun Qiao2, Fang Yuan1,3,4,5 1Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, 2School of Physics, Beijing Institute of Technology, 3Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, 4Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing Tiantan Hospital, Capital Medical University, 5China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University A protocol for the neuroprotective application of low-dose atmospheric pressure plasma treatment on glucose deprivation-induced SH-SY5Y injuries. Developmental Biology Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells Yueying Li2, Yong Hai1, Jiayu Chen3, Tie Liu1 1Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction.
