Huaqiao University 4 articles published in JoVE Biochemistry A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots Wenwu Lin*1, Hongjun Lin*2, Pengliang Xin3, Hui Yan2, Bin Kang4, Mingqing Tang2 1Emergency Department, Quanzhou First Hospital Affiliated to Fujian Medical University, 2Medicine School, Huaqiao University, 3Department of Haematology, Quanzhou First Hospital Affiliated to Fujian Medical University, 4Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University This study describes a fast and effective method for the cell component analysis of cerebral blood clots through clot dissolving, cell staining, and routine blood examination. Biochemistry Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus Mingqing Tang1,2, Hongjun Lin1, Chengjia Hu1, Hui Yan1,2 1Medicine School, Huaqiao University, 2Engineering Research Centre of Molecular Medicine of Ministry of Education Here, we present an affinity purification method of a fibrinolytic enzyme from Sipunculus nudus that is simple, inexpensive, and efficient. Bioengineering Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology Sheng-Chang Luo1,2, Ying Wang3, Ranjith Kumar Kankala1,2, Yu Shrike Zhang4, Ai-Zheng Chen1,2 1Institute of Biomaterials and Tissue Engineering, Huaqiao University, 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, 3Affiliated Dongguan Hospital, Southern Medical University, 4Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School The present protocol describes the fabrication of poly(lactic-co-glycolic acid)-based highly open porous microspheres (HOPMs) via the single-emulsion formulation based facile microfluidic technology. These microspheres have potential applications in tissue engineering and drug screening. Biology In situ Hybridization for Sipunculus nudus Coelomic Fluid Wenhua Li1, Mingrui Yuan1, Yaqin Wu1 1Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences, Huaqiao University This protocol describes an effective in situ hybridization approach to detect the mRNA expression levels and spatial patterns of target genes in Sipunculus nudus coelomic fluid.