Hunan Normal University 1 article published in JoVE Biology A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes Xinyi Liu1,2, Si Wu1,2, Wenqian Li1,2, Meihong Zhang1,2, Yan Wu1,2, Ning Zhou3, Pa Wu1,2 1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital, Central South University This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.