Hainan University 2 articles published in JoVE Biology Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing Peibo Wang*1, Yongcan Zhou*1,3, Bei Wang2, Ahmed Elaswad4, Shifeng Wang1,3, Weiliang Guo1,3, Dongdong Zhang1,3 1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, 2Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, 3Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, 4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing. Genetics An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants YueFeng Su*1,2, Rui Fan*1,3, Li-Song Hu1,4, Bao-Duo Wu1,3, Chao-Yun Hao1 1Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), 2Hainan University, 3Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, 4Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops Pinpricking the basal head of the black pepper plant is a brief and time-saving method to damage it. Here, we provided detailed steps with a video for infecting black pepper plants.