Tsinghua-Berkeley Shenzhen Institute 1 article published in JoVE Biology DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning Changyue Liu*1,2, Zhengyang Lei*1,2, Lijin Lian2, Likun Zhang1,2, Zhicheng Du1,2, Peiwu Qin1,2 1Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, 2Institute of Biopharmaceutics and Health Engineering, Tsinghua Shenzhen International Graduate School We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.
