Beijing Institute of Radiation Medicine 1 article published in JoVE Developmental Biology Generating Neural Retina from Human Pluripotent Stem Cells Wen Li*1,2, Hongyu Li*3, Hao Yan3, Lixiong Gao2, Xiaoling Wang3, Lingping Zhao3, Yuhan Yan1,2, Zi Ye2, Jiafei Xi3, Wen Yue3, Zhaohui Li1,2 1Medical School of Chinese PLA, 2Senior Department of Ophthalmology, The Third Medical Center of Chinese PLA General Hospital, 3Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine The present protocol describes an optimized 3D neural retina induction system that reduces the adhesion and fusion of retinal organoids with high repeatability and efficiency.