Takasaki University of Health and Welfare 1 article published in JoVE Immunology and Infection Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation Jun Imai1, Mayu Otani1, Takahiro Sakai1, Shinichi Hatta1 1Laboratory of Physiological Chemistry, Faculty of Pharmacy, Takasaki University of Health and Welfare The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.