Hallym University 3 articles published in JoVE Medicine In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti Jeong-Eun Park*1,2,3, Su Hoon Lee*1,2, Dong Jun Park1,2, Young Joon Seo1,2, Sung Kyun Kim3 1Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 2Research Institute of Hearing Enhancement, Yonsei University Wonju College of Medicine, 3Department of Otorhinolaryngology Head and Neck Surgery, Hallym University College of Medicine In this study, we present a real-time imaging method using confocal microscopy to observe cells moving toward damaged tissue by ex vivo incubation with the cochlear epithelium containing the organ of Corti. Genetics An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells Hyun Ah Seo1, Cho Yean Hwang1, Sokviseth Moeng1, Jong Kook Park1 1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs. Medicine A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics Natalia Petrasheskaya1, Hyun-Jin Tae1,2, Ismayil Ahmet1, Mark I. Talan1, Edward G. Lakatta1, Li Lin1 1Laboratory of Cardiovascular Sciences, National Institute on Aging, 2Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University The rat carotid balloon injury model described below allows researchers to evaluate drugs or therapeutics that negate injury-induced arterial hyperplasia. Detailed pre-surgical preparation, surgical procedure, and post-surgical cares of the animal are described.