Saevit Eye Hospital 1 article published in JoVE Genetics RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing Aeri Yoo*1, Hyun-Min Park*2, Soon-Suk Kang2, Eun-Soon Kim2, Hungwon Tchah2, Jae Yong Kim2 1Department of Ophthalmology, Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.