International University of Health and Welfare School of Medicine 1 article published in JoVE Medicine Fertility Preservation in Patients with Severe Ovarian Dysfunction Yuta Kawagoe1, Kazuhiro Kawamura1 1Advanced Reproduction Medicine Research Center, Department of Obstetrics and Gynecology, International University of Health and Welfare School of Medicine We present details of laboratory procedures for drug-free in vitro activation (IVA) of ovarian follicles for patients with severe ovarian dysfunction. This method could increase the number of retrievable oocytes per ovarian hyperstimulation and benefit fertility preservation for those patients.