Fraunhofer Institute of Interfacial Engineering and Biotechnology IGB View Institution's Website 5 articles published in JoVE Chemistry Synthesis of Soft Polysiloxane-urea Elastomers for Intraocular Lens Application Natascha Riehle*1,2, Sibylle Thude*3, Andreas Kandelbauer1,2, Günter E. M. Tovar3,4, Günter Lorenz1,2 1Reutlingen Research Institute, Reutlingen University, 2School of Applied Chemistry, Reutlingen University, 3Fraunhofer-Institute for Interfacial Engineering and Biotechnology IGB, 4Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart This study describes synthetic routes for aminopropyl-terminated polydimethylsiloxanes and polydimethyl-methyl-phenyl-siloxane-block copolymers and for soft polysiloxane-based urea (PSU) elastomers. It presents the application of PSUs as accommodating an intraocular lens. An evaluation method for in vitro cytotoxicity is also described. Bioengineering Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry Claudia Siverino1, Barbara Tabisz2, Tessa Lühmann3, Lorenz Meinel3, Thomas Müller4, Heike Walles1,2, Joachim Nickel1,2 1Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg 'Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung', Institutsteil Würzburg, 2Lehrstuhl für Tissue Engineering und Regenerative Medizin, Universitätsklinikum Würzburg, 3Lehrstuhl für Pharmazeutische Technologie und Biopharmazie, Universität Würzburg, 4Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg Biomaterials doped with Bone Morphogenetic Protein 2 (BMP2) have been used as a new therapeutic strategy to heal non-union bone fractures. To overcome side effects resulting from an uncontrollable release of the factor, we propose a new strategy to site-directly immobilize the factor, thus creating materials with improved osteogenic capabilities. Bioengineering A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds Claudia Göttlich*1, Lena C. Müller*1, Meik Kunz*3, Franziska Schmitt1, Heike Walles1,4, Thorsten Walles2, Thomas Dandekar3, Gudrun Dandekar1,4, Sarah L. Nietzer1 1Department of Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg, 2Department of Cardiothoracic Surgery, University Hospital Wuerzburg, 3Department of Bioinformatics, University Wuerzburg, 4Translational Center Wuerzburg, Fraunhofer Institute Interfacial Engineering and Biotechnology IGB We present a three-dimensional (3D) lung cancer model based on a biological collagen scaffold to study sensitivity towards non-small-cell-lung-cancer-(NSCLC)-targeted therapies. We demonstrate different read-out techniques to determine the proliferation index, apoptosis and epithelial-mesenchymal transition (EMT) status. Collected data are integrated into an in silico model for prediction of drug sensitivity. Bioengineering Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding Angela Rossi*1, Antje Appelt-Menzel*1, Szymon Kurdyn1, Heike Walles1,2, Florian Groeber2 1Department for Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 2Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease, Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility. Bioengineering Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy Miriam Votteler1,2, Daniel A. Carvajal Berrio2, Marieke Pudlas2,3, Heike Walles2,4, Katja Schenke-Layland1,2 1Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard Karls University, Tübingen, 2Department of Cell and Tissue Engineering, Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, 3Department for Medical Interfacial Engineering (IGVT), University of Stuttgart, Germany, 4Institute of Tissue Engineering and Regenerative Medicine, Julius-Maximillians University, Würzburg, Germany Raman spectroscopy is a suitable technique for the non-contact, label-free analysis of living cells, tissue-engineered constructs and native tissues. Source-specific spectral fingerprints can be generated and analyzed using multivariate analysis.