Medical Laser Center Luebeck GmbH, University of Leubeck 1 article published in JoVE Biology Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects Yoko Miura1,2,3, Joachim Pruessner1, Carla Lotta Mertineit1, Katharina Kern1, Michael Muenter1, Moritz Moltmann2, Veit Danicke2, Ralf Brinkmann1,2 1Institute of Biomedical Optics, University of Luebeck, 2Medical Laser Center Luebeck GmbH, University of Leubeck, 3Department of Ophthalmology, University of Luebeck An original experimental setup for heating cells in a culture dish using 1.94 µm continuous-wave laser radiation is introduced here. Using this method, the biological responses of retinal pigment epithelial (RPE) cells after different thermal exposures can be investigated.