Buck GmbH and Co.KG 1 article published in JoVE Bioengineering Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant Gauri Tendulkar1, Phillip Grau1, Patrick Ziegler1,2, Alfred Buck, Sr.3, Alfred Buck, Jr.3, Andreas Badke1,2, Hans-Peter Kaps1,2, Sabrina Ehnert1, Andreas K. Nussler1 1Siegfried Weller Institute for Trauma Research at the BG Trauma Center, Eberhard Karls Universität Tübingen, 2Department of Orthopaedics, BG Trauma-Center, 3Buck GmbH and Co.KG Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.