Mundomar, Benidorm 1 article published in JoVE Developmental Biology Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization María Jesús Sánchez-Calabuig1,2, Francisco Alberto García-Vázquez3, Ricardo Laguna-Barraza1, Carlos Barros-García4, Daniel García-Parraga5, Dimitrios Rizos1, Alfonso Gutiérrez Adan1, José Félix Pérez-Gutíerrez2 1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5 Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.