De Montfort University View Institution's Website 3 articles published in JoVE Biology A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage Yunhee Ji*1, Mahsa Karbaschi*2, Abdulhadi Abdulwahed*3, Natalia S. Quinete4, Mark D. Evans5, Marcus S. Cooke1 1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University The comet assay is a popular means of detecting DNA damage. This study describes an approach to running slides in representative variants of the comet assay. This approach significantly increased the number of samples while decreasing assay run-time, the number of slide manipulations, and the risk of damage to gels. Engineering Inkjet-printed Polyvinyl Alcohol Multilayers Iulia Salaoru1,2, Zuoxin Zhou2, Peter Morris3, Gregory J. Gibbons2 1Emerging Technologies Research Centre (EMTERC), De Montfort University, 2WMG, University of Warwick, 3PVOH Polymer Ltd. An inkjet printer was used to manufacture polyvinyl alcohol multilayers. Polyvinyl alcohol water-based ink was formulated, and the main physical properties were investigated. Behavior Simultaneous EEG Monitoring During Transcranial Direct Current Stimulation Pedro Schestatsky1,2,3, Leon Morales-Quezada3,4, Felipe Fregni3 1Programa de Pós-Graduação em Ciências Médica, Universidade Federal do Rio Grande do Sul, 2Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), 3Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 4De Montfort University Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique that has shown initial therapeutic effects in several neurological conditions. The main mechanism underlying these therapeutic effects is the modulation of cortical excitability. Therefore, online monitoring of cortical excitability would help guide stimulation parameters and optimize its therapeutic effects. In the present article we review the use of a novel device that combines simultaneous tDCS and EEG monitoring in real time.